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PKC alpha 抗体 (N-Term)

Name: PKC alpha 抗体 (N-Term)
SKU: ABIN3043025
Availability: OutOfStock

Cited in 5+ publications. This 兔多克隆 anti-PKC alpha antibody specifically detects PKC alpha in WB 和 IHC. The antibody is reactive with 人, 小鼠和大鼠 samples.

Anti-PKC alpha antibody, Western blotting WB: HELA Cell Lysate (PKC alpha 抗体 (N-Term))

Anti-PKC alpha antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue (PKC alpha 抗体 (N-Term))

IHC

3 images

(5 references)

产品编号 ABIN3043025

发货至: 中国

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中国
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Quick Overview for PKC alpha 抗体 (N-Term) (ABIN3043025)

抗原

See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This PKC alpha antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product PKCa Primary Antibody

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AA 153-182, N-Term

原理

Anti-PKC alpha/PRKCA Antibody

序列

MDHTEKRGRI YLKAEVADEK LHVTVRDAKN

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-PKC alpha/PRKCA Antibody (ABIN3043025). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA, different from the related rat and mouse sequences by one amino acid.

亚型

IgG
anti-Protein Kinase C, alpha (PKCa) antibody
KD Validated PKCa 适用: 人 WB, ICC, FACS, IF (p) 宿主: 兔 Monoclonal 23GB1690 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (AA 563-672) antibody
PKCa 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 2F11 unconjugated

anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibody
PKCa 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated

anti-Protein Kinase C, alpha (PKCa) (pThr638) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (pSer657) antibody
KD Validated PKCa 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB1055 unconjugated Recombinant Antibody

anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibody
PKCa 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB01012 unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
PKCa 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) antibody
KD Validated PKCa 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB4475 unconjugated Recombinant Antibody

应用备注

Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Yan-Fang, Zhi-Heng, Li-Xiao, Fang, Jun, Gang, Lan, Na-Na, Xiao-Juan, Li-Chao, Wen-Li, Pei-Fang, He, Guang-Hao, Yan-Hong, Yi-Ping, Yun-Yun, Hui-Ting, Yi, Mei-Fang, Lin, Jian, Shao-Yan, Xue-Ming, Xing et al.: "Molecular Mechanism of the Cell Death Induced by the Histone Deacetylase Pan Inhibitor LBH589 (Panobinostat) in Wilms Tumor Cells. ..." in: PLoS ONE, Vol. 10, Issue 7, pp. e0126566, (2016) (PubMed).

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Cheng, Gu, Zhang, Yuan, Zhao, Jiang, Jia: "Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-?-ERK1/2-NF-?B pathway." in: International immunopharmacology, Vol. 23, Issue 1, pp. 304-13, (2015) (PubMed).
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PRKCA

背景

Synonyms: Protein kinase C alpha type,PKC-A,PKC-alpha,2.7.11.13,PRKCA,PKCA, PRKACA,
Tissue Specificity: Brain and peripheral tissues.
Background: Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.
Sequence Similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

分子量

77 kDa

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling