BID 抗体 (C-Term)
(1 reference)Quick Overview for BID 抗体 (C-Term) (ABIN3042950)
抗原
See all BID 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 179-195, C-Term
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原理
- Anti-Bid Antibody Picoband®
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序列
- NQNLFSYVRN LVRNEMD
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Bid Antibody (ABIN3042950). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid, different from the related rat sequence by one amino acid.
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亚型
- IgG
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anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
BID 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) antibodyBID 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3C5 unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 68-103) antibodyBID 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB0957-RB0958 unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-196) antibodyBID 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-130) antibodyBID 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibodyBID 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 68-103) antibodyBID 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibodyBID 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibodyBID 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F3-1A3 unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 39-68) antibodyBID 适用: 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat By Heat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. 2. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. 3. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Sonodynamically induced apoptosis by protoporphyrin IX on hepatoma-22 cells in vitro." in: Ultrasound in medicine & biology, Vol. 36, Issue 4, pp. 667-76, (2010) (PubMed).
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: "Sonodynamically induced apoptosis by protoporphyrin IX on hepatoma-22 cells in vitro." in: Ultrasound in medicine & biology, Vol. 36, Issue 4, pp. 667-76, (2010) (PubMed).
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- BID (BH3 Interacting Domain Death Agonist (BID))
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别名
- Bid
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背景
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Synonyms: BH3-interacting domain death agonist,p22 BID,BID,BH3-interacting domain death agonist p15,p15 BID,BH3-interacting domain death agonist p13,p13 BID,BH3-interacting domain death agonist p11,p11 BID,Bid,
Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.
Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Sequence Similarities: Contains 1 C1q domain.
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分子量
- 22 kDa
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UniProt
- P70444
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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