COX1 抗体 (N-Term)
Quick Overview for COX1 抗体 (N-Term) (ABIN3042632)
抗原
See all COX1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-14, N-Term
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原理
- Anti-MTCO1/MT-CO1 Antibody Picoband®
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序列
- FADRWLFSTN HKD
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MTCO1/MT-CO1 Antibody (ABIN3042632). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human MTCO1.
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亚型
- IgG
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anti-Cytochrome C Oxidase Subunit I (COX1) (AA 318-599) antibody
COX1 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (AA 401-500) antibodyCOX1 适用: 小鼠, 大鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (AA 474-513) antibodyCOX1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (AA 1-160) antibodyCOX1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (C-Term) antibodyCOX1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (AA 195-224) antibodyCOX1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB24524 unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (C-Term) antibodyCOX1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome C Oxidase Subunit I (COX1) (AA 550-599) antibodyCOX1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
1. Kadenbach, B., Jarausch, J., Hartmann, R., Merle, P. : Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure. Anal. Biochem. 129: 517-521, 1983. 2. Shoffner, J. M., Wallace, D. C. : Oxidative phosphorylation diseases.In: Scriver, C. R., Beaudet, A. L., Sly, W. S., Valle, D. (eds.) : The Metabolic and Molecular Bases of Inherited Disease. Vol. 1. New York: McGraw-Hill (7th ed.) 1995. Pp. 1535-1609. 3. Hosler, J. P., Ferguson-Miller, S., Calhoun, M. W., Thomas, J. W., Hill, J., Lemieux, L., Ma, J., Georgiou, C., Fetter, J., Shapleigh, J., Tecklenburg, M. M. J., Babcock, G. T., Gennis, R. B. : Insight into the active-site structure and function of cytochrome oxidase by analysis of site-ed mutants of bacterial cytochrome aa3 and cytochrome bo. J. Bioenerg. Biomembr. 25: 121-136, 1993. 4. Acin-Perez, R., Bayona-Bafaluy, M. P., Bueno, M., Machicado, C., Fernandez-Silva, P., Perez-Martos, A., Montoya, J., Lopez-Perez, M. J., Sancho, J., Enriquez, J. A. : An intragenic suppressor in the cytochrome c oxidase I gene of mouse mitochondrial DNA. Hum. Molec. Genet. 12: 329-339, 2003. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- COX1 (Cytochrome C Oxidase Subunit I (COX1))
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别名
- MT-CO1
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背景
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Synonyms: Cytochrome c oxidase subunit 1,1.9.3.1,Cytochrome c oxidase polypeptide I,MT-CO1,COI, COXI, MTCO1,
Tissue Specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Background: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
Sequence Similarities: Belongs to the SNAP-25 family.
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分子量
- 37 kDa
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UniProt
- P00395
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途径
- Proton Transport
抗原
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