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S100A8 抗体 (AA 2-89)

This anti-S100A8 antibody is a 兔 多克隆 antibody detecting S100A8 in WB, IHC, ICC, IF 和 FACS. Suitable for 小鼠 和 大鼠.
产品编号 ABIN3042481
发货至: 中国

Quick Overview for S100A8 抗体 (AA 2-89) (ABIN3042481)

抗原

See all S100A8 抗体
S100A8 (S100 Calcium Binding Protein A8 (S100A8))

适用

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小鼠, 大鼠

宿主

  • 118
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克隆类型

  • 120
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多克隆

标记

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This S100A8 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 抗原表位

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    AA 2-89

    原理

    Anti-MRP8/S100A8 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with recombinant human S100A8 is observed.

    产品特性

    Anti-MRP8/A8 Antibody Picoband® (ABIN3042481). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Mouse
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
    1. "Entrez Gene: S100A8 S100 calcium binding protein A8". 2. Kerkhoff C, Klempt M, Sorg C (1999). "Novel insights into structure and function of MRP8 (S100A8) and MRP14 (S100A9).". Biochim. Biophys. Acta 1448 (2): 200-11. 3. Schäfer T, Sachse GE, Gassen HG (1991). "The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells.". Biol. Chem. Hoppe-Seyler 372 (1): 1-4.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    S100A8 (S100 Calcium Binding Protein A8 (S100A8))

    别名

    S100a8

    背景

    Synonyms: Protein S100-A8,Calgranulin-A,Chemotactic cytokine CP-10,Leukocyte L1 complex light chain,Migration inhibitory factor-related protein 8,MRP-8,p8,Pro-inflammatory S100 cytokine,S100 calcium-binding protein A8,S100a8,Caga, Mrp8,

    Tissue Specificity: Ubiquitously expressed, with highest levels in skeletal muscle, heart and thymus. Overexpressed in prostate tumor tissue. .

    Background: S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

    分子量

    11 kDa

    基因ID

    20201

    UniProt

    P27005

    途径

    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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