HSP27 抗体 (AA 1-205)
Quick Overview for HSP27 抗体 (AA 1-205) (ABIN3042464)
抗原
See all HSP27 (HSPB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-205
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原理
- Anti-Hsp27/HSPB1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Hsp27/HSPB1 Antibody Picoband® (ABIN3042464). Tested in Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
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亚型
- IgG
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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, FACS, IP 宿主: 兔 Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 小鼠, 大鼠, 绵羊, Chimpanzee, 小鸡, 猴 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal G3-1 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 小鼠 Monoclonal 5D12-A12 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibodyHSPB1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, -
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/mLx106 cells, Human, -
1. Ackerley, S., James, P. A., Kalli, A., French, S., Davies, K. E., Talbot, K. A mutation in the small heat-shock protein HSPB1 leading to distal hereditary motor neuronopathy disrupts neurofilament assembly and the axonal transport of specific cellular cargoes. Hum. Molec. Genet. 15: 347-354, 2006. 2. d'Ydewalle, C., Krishnan, J., Chiheb, D. M., Van Damme, P., Irobi, J., Kozikowski, A. P., Vanden Berghe, P., Timmerman, V., Robberecht, W., Van Den Bosch, L. HDAC6 inhibitors reverse axonal loss in a mouse model of mutant HSPB1-induced Charcot-Marie-Tooth disease. Nature Med. 17: 968-974, 2011. 3. Stock, A. D., Spallone, P. A., Dennis, T. R., Netski, D., Morris, C. A., Mervis, C. B., Hobart, H. H. Heat shock protein 27 gene: chromosomal and molecular location and relationship to Williams syndrome. Am. J. Med. Genet. 120A: 320-325, 2003. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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别名
- HSPB1
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背景
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Synonyms: Heat shock protein beta-1,HspB1,28 kDa heat shock protein,Estrogen-regulated 24 kDa protein,Heat shock 27 kDa protein,HSP 27,Stress-responsive protein 27,SRP27,HSPB1,HSP27, HSP28,
Tissue Specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .
Background: HSPB1 (Heat shock 27 kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Sequence Similarities: Belongs to the small heat shock protein (HSP20) family.
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分子量
- 27 kDa
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基因ID
- 3315
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UniProt
- P04792
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途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
抗原
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