Gelsolin 抗体 (AA 580-782)
(1 reference)Quick Overview for Gelsolin 抗体 (AA 580-782) (ABIN3042436)
抗原
See all Gelsolin (GSN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 580-782
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原理
- Anti-Gelsolin/GSN Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Gelsolin/GSN Antibody Picoband® (ABIN3042436). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Gelsolin recombinant protein (Position: E580-A782). Human Gelsolin shares 94% and 95% amino acid (aa) sequences identity with mouse and rat Gelsolin, respectively.
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亚型
- IgG
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anti-Gelsolin (GSN) (AA 170-423) antibody
GSN 适用: Cow WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Gelsolin (GSN) (AA 577-771) antibodyGSN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gelsolin (GSN) (AA 674-782) antibodyGSN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gelsolin (GSN) (AA 673-782) antibodyGSN 适用: 人 WB, IP, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G5 unconjugated
anti-Gelsolin (GSN) (AA 543-782) antibodyGSN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Gelsolin (GSN) antibodyGSN 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5E3B9 unconjugated
anti-Gelsolin (GSN) (AA 432-553) antibodyGSN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Gelsolin (GSN) (AA 432-625) antibodyGSN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gelsolin (GSN) (AA 1-782) antibodyGSN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Gelsolin (GSN) (C-Term) antibodyKO Validated GSN 适用: 人 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Kamada, S., Kusano, H., Fujita, H., Ohtsu, M., Koya, R. C., Kuzumaki, N., Tsujimoto, Y. A cloning method for caspase substrates that uses the yeast two-hybrid system: cloning of the antiapoptotic gene gelsolin. Proc. Nat. Acad. Sci. 95: 8532-8537, 1998. 2. Kim, J., Lee, J. E., Heynen-Genel, S., Suyama, E., Ono, K., Lee, K., Ideker, T., Aza-Blanc, P., Gleeson, J. G. Functional genomic screen for modulators of ciliogenesis and cilium length. Nature 464: 1048-1051, 2010. 3. Vasconcellos, C. A., Allen, P. G., Wohl, M. E., Drazen, J. M., Janmey, P. A., Stossel, T. P. Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin. Science 263: 969-971, 1994. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Proteomics of acute heart failure in a rat post-myocardial infarction model." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).
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: "Proteomics of acute heart failure in a rat post-myocardial infarction model." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).
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- Gelsolin (GSN)
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别名
- GSN
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背景
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Synonyms: Gelsolin,AGEL,Actin-depolymerizing factor,ADF,Brevin,GSN,
Tissue Specificity: Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Background: Gelsolin, also known as GNS or brevin, is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. It is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.
Sequence Similarities: Belongs to the villin/gelsolin family.
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分子量
- 86 kDa
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基因ID
- 2934
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UniProt
- P06396
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途径
- Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy
抗原
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