Flotillin 2 抗体 (AA 169-344)
Quick Overview for Flotillin 2 抗体 (AA 169-344) (ABIN3042393)
抗原
See all Flotillin 2 (FLOT2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 169-344
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原理
- Anti-Flotillin 2/FLOT2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Flotillin 2/FLOT2 Antibody Picoband® (ABIN3042393). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Flotillin 2 recombinant protein (Position: K169-K344). Human Flotillin 2 shares 100% amino acid (aa) sequence identity with both mouse and rat Flotillin 2.
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亚型
- IgG
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anti-Flotillin 2 (FLOT2) (C-Term) antibody
FLOT2 适用: 人 WB, IHC (p), IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 1-379) antibodyFLOT2 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3G6 unconjugated
anti-Flotillin 2 (FLOT2) (AA 1-379) antibodyFLOT2 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 239-428) antibodyFLOT2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (C-Term) antibodyVerified FLOT2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 1-379) antibodyFLOT2 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) antibodyFLOT2 适用: 化学剂 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) antibodyFLOT2 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 2 (FLOT2) (AA 322-356) antibodyFLOT2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB56189 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. 2. Li, Y., Martin, B. R., Cravatt, B. F., Hofmann, S. L. DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells. J. Biol. Chem. 282: 523-530, 2012. 3. Schroeder, W. T., Siciliano, M. J., Stewart-Galetka, S. L., Duvic, M. The human gene for an epidermal surface antigen (M17S1) is located at 17q11-12. Genomics 11: 481-482, 1991. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Flotillin 2 (FLOT2)
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别名
- FLOT2
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背景
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Synonyms: Flotillin-2,Epidermal surface antigen,ESA,Membrane component chromosome 17 surface marker 1,FLOT2,ESA1, M17S1,
Tissue Specificity: In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Background: FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.
Sequence Similarities: Contains 1 HMG box DNA-binding domain.
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分子量
- 47-50 kDa
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基因ID
- 2319
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UniProt
- Q14254
抗原
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