BLM 抗体 (AA 1319-1335)
Quick Overview for BLM 抗体 (AA 1319-1335) (ABIN303411)
抗原
See all BLM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1319-1335
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特异性
- Recognises Bloom's Syndrome Protein (BLM).
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纯化方法
- Protein G Chromatography.
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免疫原
- Synthetic Peptide - KLH conjugated corresponding to Amino acids 1319 to 1335 of Human Bloom Syndrome protein (BLM).
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anti-Bloom Syndrome RecQ Like Helicase (BLM) (C-Term) antibody
BLM 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Bloom Syndrome RecQ Like Helicase (BLM) (pThr99) antibodyBLM 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bloom Syndrome RecQ Like Helicase (BLM) (AA 1196-1295) antibodyBLM 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E4 unconjugated
anti-Bloom Syndrome RecQ Like Helicase (BLM) (N-Term) antibodyBLM 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Bloom Syndrome RecQ Like Helicase (BLM) (AA 1196-1295) antibodyBLM 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Bloom Syndrome RecQ Like Helicase (BLM) (AA 1054-1082), (C-Term) antibodyBLM 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB25943 unconjugated
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应用备注
- ELISA (1/0-1/1000). Immunohistochemistry on Paraffin Sections (10 μg/mL).
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限制
- 仅限研究用
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缓冲液
- Phosphate Buffered Saline PBS containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid cycles of freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.
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- BLM (Bloom Syndrome RecQ Like Helicase (BLM))
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别名
- BLM
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背景
- The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44 % amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions. The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process. Bloom syndrome cells show marked genomic instability, in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.Synonyms: Bloom syndrome protein, DNA helicase, RECQ2, RECQL3, RecQ protein-like 3, RecQ-like type 2
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基因ID
- 641
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NCBI登录号
- NP_000048
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UniProt
- P54132
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途径
- DNA Damage Repair
抗原
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