MBD4 抗体 (C-Term)
Quick Overview for MBD4 抗体 (C-Term) (ABIN3031772)
抗原
See all MBD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the C-terminus of human Methyl-CpG-Binding Domain Protein 4 (YHDWLWENHEKLSLS) was used as the immunogen for this MBD4 antibody (100% homologous in human, mouse and rat).
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亚型
- IgG
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anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 323-351) antibody
MBD4 适用: 人 WB 宿主: 兔 Polyclonal RB40399 unconjugated
anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 566-580), (C-Term) antibodyMBD4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (Middle Region) antibodyMBD4 适用: 人, 豚鼠 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the MBD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the MBD4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
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别名
- MBD4
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背景
- Methyl-CpG-Binding Domain Protein 4, also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and -4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and -4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
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UniProt
- O95243
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途径
- DNA Damage Repair
抗原
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