MBD4 抗体 (C-Term)
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Quick Overview for MBD4 抗体 (C-Term) (ABIN3044062)
抗原
See all MBD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-MBD4 Antibody
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序列
- YHDWLWENHE KLSLS
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins
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预测反应
- identical to the related rat,mouse sequences.
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产品特性
- Anti-MBD4 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 16-580) antibody
MBD4 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 323-351) antibodyMBD4 适用: 人 WB 宿主: 兔 Polyclonal RB40399 unconjugated
anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 1-50) antibodyMBD4 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 1-50) antibodyMBD4 适用: 人 WB, IHC (fp), IP 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 4 (MBD4) (Middle Region) antibodyMBD4 适用: 人, 豚鼠 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))
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别名
- MBD4
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背景
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Background: MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
Gene Full Name: methyl-CpG binding domain 4, DNA glycosylase
Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.
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分子量
- 80 kDa
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基因ID
- 8930
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UniProt
- O95243
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途径
- DNA Damage Repair
抗原
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