beta Actin 抗体 (AA 2-19)
Quick Overview for beta Actin 抗体 (AA 2-19) (ABIN3029836)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-19
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纯化方法
- Antigen affinity
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免疫原
- Amino acids 2-19 (DDDIAALVVDNGSGMCKA) were used as the immunogen for this b-Actin antibody (100% homologous in human, mouse and rat).
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亚型
- IgG
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anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人 WB, IF, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Actin, beta (ACTB) (AA 2-16) antibodyACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 350-375) antibodyACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 1-25) antibodyACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated
anti-Actin, beta (ACTB) (AA 1-375) antibodyACTB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) (AA 1-375) antibodyACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated
anti-Actin, beta (ACTB) (N-Term) antibodyKO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated
anti-Actin, beta (ACTB) antibodyACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated
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应用备注
- The stated application concentrations are suggested starting points. Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the b-Actin antibody can be stored for up to one month at 4 °C. For long-term, aliquot and store at -20 °C. Avoid repeated freezing and thawing.
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- B-Actin
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背景
- B-Actin (ACTB) is one of six different isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal isoforms. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al.(1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped four ACTB pseudogenes to other chromosomes. Karakozova et al.(2006) found that arginylation of b-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al.(2006)concluded that arginylation of b-actin apparently represents a critical step in the actin N-terminal processing needed for functioning in vivo.
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UniProt
- P60709
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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