MUC5AC 抗体
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北京 101111
Quick Overview for MUC5AC 抗体 (ABIN3026776)
抗原
See all MUC5AC 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- MUC5AC Antibody / Mucin-5AC (azide and preservative free)
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无交叉反应
- 牛
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产品特性
- This antibody recognizes the peptide core of gastric mucin M1 (>1,000 kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
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纯化方法
- Protein G affinity chromatography
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免疫原
- M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.
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亚型
- IgG1, kappa
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anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 5528-5627) antibody
MUC5AC 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 8H8G6 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 28-327) antibodyMUC5AC 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 6C9D11 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) antibodyMUC5AC 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal 45M1 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 5528-5627) antibodyMUC5AC 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7E9D8 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 4848-5030) antibodyMUC5AC 适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 601-700) antibodyMUC5AC 适用: 人, 小鼠, 大鼠 ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 430-549) antibodyMUC5AC 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (AA 351-450) antibodyMUC5AC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H7 unconjugated
anti-Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC) (C-Term) antibodyMUC5AC 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the MUC5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
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别名
- MUC5AC
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背景
- This antibody recognizes the peptide core of gastric mucin M1 (>1,000 kDa), now identified as Mucin-5AC or MUC5AC. The MUC5AC epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. MUC5AC antibody reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
抗原
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