HSP27 抗体
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- 抗原 See all HSP27 (HSPB1) 抗体
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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适用
- 人, 大鼠, 小鼠, 绵羊, 小鸡, Chimpanzee
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSP27 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced "p29" and "24K" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
- 克隆位点
- G3-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product HSPB1 Primary Antibody
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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, FACS, IP 宿主: 兔 Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠, 小鼠, 绵羊, 猴, 小鸡, Chimpanzee WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal G3-1 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 小鼠 Monoclonal 5D12-A12 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 HRP
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- 别名
- HSP27 (HSPB1 产品)
- 别名
- CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody
- 背景
- This antibody recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced ""p29"" and ""24K"" protein. About 50 % of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
- 途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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