TGFA 抗体 (AA 34-43)
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北京 101111
Quick Overview for TGFA 抗体 (AA 34-43) (ABIN3026449)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 34-43
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产品特性
- This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.
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亚型
- IgG1 kappa
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anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibody
TGFA 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal D20 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibodyTGFA 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 118-150) antibodyTGFA 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB56968 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 1-160) antibodyTGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (C-Term) antibodyTGFA 适用: 人, 大鼠, 小鼠, Cow IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MF9 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人 IHC, WB, ELISA, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the TGF-alpha antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the TGF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF-alpha
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背景
- This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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