CEA 抗体
Quick Overview for CEA 抗体 (ABIN3026334)
抗原
See all CEA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody cocktail recognizes proteins of 80-200 kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody cocktail shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Human colon carcinoma extract (COL-1 & CEA31) and CEA recombinant protein (C66/261) were used as the immunogen for this antibody cocktail.
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亚型
- IgG2a
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anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 448-572) antibody
CEA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 WB, ELISA, IHC (p), IF (p) 宿主: 小鼠 Monoclonal B5 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 315-365) antibodyCEA 适用: 人 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1C7 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 3G12 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 400-429) antibodyCEA 适用: 人 WB, IHC, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 ELISA, IHC (p), IF (p), IF (cc) 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) antibodyCEA 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-465R unconjugated Recombinant Antibody
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 566-698) antibodyCEA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carcinoembryonic Antigen Gene Family (CEA) (AA 544-771) antibodyCEA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CEA antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CEA (Carcinoembryonic Antigen Gene Family (CEA))
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别名
- CEA
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背景
- This antibody cocktail recognizes proteins of 80-200 kDa, identified as different members of the CEA (Carcinoembryonic Antigen) family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. The antibody cocktail shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). CEA antibody positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
抗原
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