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TGFA 抗体 (AA 34-43)

This anti-TGFA antibody is a 小鼠 单克隆 antibody detecting TGFA in IF, FACS 和 IHC (p). Suitable for 人, 兔 和 斑马鱼.
产品编号 ABIN3026164
发货至: 中国

Quick Overview for TGFA 抗体 (AA 34-43) (ABIN3026164)

抗原

See all TGFA 抗体
TGFA (Transforming Growth Factor, alpha (TGFA))

适用

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  • 37
  • 31
  • 18
  • 16
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  • 1
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  • 1
人, 兔, 斑马鱼

宿主

  • 77
  • 57
  • 4
  • 3
  • 2
小鼠

克隆类型

  • 75
  • 67
  • 1
单克隆

标记

  • 84
  • 11
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
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  • 2
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This TGFA antibody is un-conjugated

应用范围

  • 79
  • 76
  • 43
  • 36
  • 34
  • 34
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  • 11
  • 9
  • 4
  • 4
  • 3
  • 3
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  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

1E8-G6
  • 抗原表位

    • 21
    • 18
    • 13
    • 11
    • 3
    • 2
    • 2
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    AA 34-43

    纯化方法

    PEG precipitation

    免疫原

    A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

    亚型

    IgM kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    TGFA (Transforming Growth Factor, alpha (TGFA))

    别名

    TGF alpha

    背景

    TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

    基因ID

    7039

    途径

    NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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