TGFA 抗体 (AA 34-43)
Quick Overview for TGFA 抗体 (AA 34-43) (ABIN3026164)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 34-43
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纯化方法
- PEG precipitation
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免疫原
- A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.
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亚型
- IgM kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF alpha
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背景
- TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
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基因ID
- 7039
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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