TGFA 抗体 (C-Term)
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Quick Overview for TGFA 抗体 (C-Term) (ABIN3026162)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- C-Term
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原理
- TGF alpha Antibody (azide and preservative free)
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纯化方法
- Protein G affinity chromatography
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免疫原
- A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
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亚型
- IgG1, kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissues requires boiling sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF alpha
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背景
- TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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