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Prostate Specific Antigen 抗体

The 小鼠 单克隆 anti-Prostate Specific Antigen antibody (Clone 1A7 or 1A7G6B6) (ABIN3026041) specifically detects Prostate Specific Antigen in IHC (p), IF 和 FACS. The antibody is reactive with 人 和 Cat samples.
产品编号 ABIN3026041
发货至: 中国
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Quick Overview for Prostate Specific Antigen 抗体 (ABIN3026041)

抗原

See all Prostate Specific Antigen (PSA) 抗体
Prostate Specific Antigen (PSA)

适用

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人, Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This Prostate Specific Antigen antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

1A7 or 1A7G6B6
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    PSA (p30) from human sperm plasma was used as the immunogen for the Prostate Specific Antigen antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the Prostate Specific Antigen antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Prostate Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Prostate Specific Antigen (PSA)

    别名

    Prostate Specific Antigen

    背景

    Recognizes a single protein of 33-34  kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This mAb makes an excellent pair with mAb A67-B/E3 for PSA tests.

    途径

    Complement System
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