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Prostate Specific Antigen 抗体

The 小鼠 单克隆 anti-Prostate Specific Antigen antibody is suitable to detect Prostate Specific Antigen in samples from 人. It has been validated for IHC (p), IF 和 FACS.
产品编号 ABIN3026028
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Prostate Specific Antigen 抗体 (ABIN3026028)

抗原

See all Prostate Specific Antigen (PSA) 抗体
Prostate Specific Antigen (PSA)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Prostate Specific Antigen antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

A67-B-E3-1A7
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    PSA (p30) from human sperm plasma was used as the immunogen for the Prostate Specific Antigen antibody cocktail.

    亚型

    IgG
  • 应用备注

    Optimal dilution of the Prostate Specific Antigen antibody cocktail should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Prostate Specific Antigen antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Prostate Specific Antigen (PSA)

    别名

    Prostate Specific Antigen

    背景

    Recognizes a single protein of 33-34  kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

    途径

    Complement System
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