GRP94 抗体
Quick Overview for GRP94 抗体 (ABIN3025969)
抗原
See all GRP94 (HSP90B1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.
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亚型
- IgG2a kappa
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
HSP90B1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibodyHSP90B1 适用: 人, 小鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibodyVerified HSP90B1 适用: 人, 小鼠 WB, IHC, FACS, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 WB, IHC, FACS, IF, StM 宿主: 大鼠 Monoclonal 9G10-F8-2 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 IHC, FACS, StM 宿主: 兔 Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibodyHSP90B1 适用: 人, 大鼠 WB, IHC, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, FACS, IF, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3 unconjugated
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应用备注
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Optimal dilution of the GRP94 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistology (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
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别名
- GRP94
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背景
- Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
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途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
抗原
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