Prostate Specific Antigen 抗体
Quick Overview for Prostate Specific Antigen 抗体 (ABIN3025436)
抗原
See all Prostate Specific Antigen (PSA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G purified
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免疫原
- Full length human recombinant protein was used as the immunogen for this PSA antibody.
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亚型
- IgG1 kappa
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应用备注
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Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: For coating use Ab at 1-5 μg/mL, order Ab without BSA,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the PSA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Prostate Specific Antigen (PSA)
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别名
- PSA
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背景
- Prostate specific antigen / PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. Using a Prostate specific antigen antibody, the protein can be detected in the sera of patients with prostatic carcinoma and expression level correlates positively with the likelyhood of cancer.
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基因ID
- 354
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途径
- Complement System
抗原
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