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POMC 抗体 (N-Term)

This anti-POMC antibody is a 小鼠 单克隆 antibody detecting POMC in FACS, IF 和 IHC (p). Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN3025418
发货至: 中国
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Quick Overview for POMC 抗体 (N-Term) (ABIN3025418)

抗原

See all POMC 抗体
POMC (Proopiomelanocortin (POMC))

适用

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人, 小鼠, 大鼠

宿主

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  • 32
  • 1
小鼠

克隆类型

  • 52
  • 43
单克隆

标记

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This POMC antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

57
  • 抗原表位

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    N-Term

    纯化方法

    Protein G affinity chromatography

    免疫原

    An N-terminal peptide from the ACTH region of human POMC was used as the immunogen for this POMC antibody.

    亚型

    IgG1
  • 应用备注

    The optimal dilution of the ACTH antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the POMC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    POMC (Proopiomelanocortin (POMC))

    别名

    POMC

    背景

    POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.

    途径

    Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour
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