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PODXL 抗体 (Extracellular Domain, Internal Region)

This anti-PODXL antibody is a 小鼠 单克隆 antibody detecting PODXL in FACS, IF 和 IHC (p). Suitable for 人, 大鼠 和 兔.
产品编号 ABIN3025409
发货至: 中国

Quick Overview for PODXL 抗体 (Extracellular Domain, Internal Region) (ABIN3025409)

抗原

See all PODXL 抗体
PODXL (Podocalyxin-Like (PODXL))

适用

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  • 27
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  • 1
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人, 大鼠, 兔

宿主

  • 68
  • 55
  • 1
小鼠

克隆类型

  • 70
  • 54
单克隆

标记

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  • 2
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  • 2
  • 2
  • 2
  • 2
  • 1
This PODXL antibody is un-conjugated

应用范围

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  • 1
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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

2A4
  • 抗原表位

    • 23
    • 16
    • 9
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain, Internal Region

    纯化方法

    PEG precipitation

    免疫原

    A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL was used as the immunogen for the Podocalyxin antibody.

    亚型

    IgM
  • 应用备注

    Optimal dilution of the Podocalyxin antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    PODXL (Podocalyxin-Like (PODXL))

    别名

    Podocalyxin

    背景

    Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

    途径

    Tube Formation
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