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PODXL 抗体 (Extracellular Domain)

PODXL 适用: 人, 大鼠, 兔 WB, FACS, IP, IF, IHC (p) 宿主: 小鼠 Monoclonal 3D3 unconjugated
产品编号 ABIN3025406
发货至: 中国
  • 抗原 See all PODXL 抗体
    PODXL (Podocalyxin-Like (PODXL))
    抗原表位
    • 15
    • 13
    • 12
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    适用
    • 81
    • 22
    • 13
    • 8
    • 1
    • 1
    人, 大鼠, 兔
    宿主
    • 52
    • 39
    • 1
    小鼠
    克隆类型
    • 53
    • 39
    单克隆
    标记
    • 44
    • 8
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PODXL antibody is un-conjugated
    应用范围
    • 66
    • 32
    • 29
    • 27
    • 21
    • 19
    • 9
    • 9
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
    克隆位点
    3D3
    亚型
    IgG1
    Top Product
    Discover our top product PODXL Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IP: 1-2 μg/500 μg protein,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    PODXL (Podocalyxin-Like (PODXL))
    别名
    Podocalyxin (PODXL 产品)
    别名
    Gp200 antibody, PC antibody, PCLP antibody, PCLP-1 antibody, PCB antibody, AW121214 antibody, Ly102 antibody, Pclp1 antibody, podocalyxin antibody, MEP21 antibody, PODXL antibody, Gp135 antibody, PCLP1 antibody, cPCLP1 antibody, podxl antibody, fc18d02 antibody, wu:fc18d02 antibody, Pc antibody, Pcb antibody, Podxl antibody, podocalyxin like antibody, pyruvate carboxylase antibody, podocalyxin-like antibody, PODXL antibody, PC antibody, Podxl antibody, podxl antibody, Pcx antibody
    背景
    Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
    基因ID
    5420
    途径
    Tube Formation
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