Laminin gamma 1 抗体
Quick Overview for Laminin gamma 1 抗体 (ABIN3025186)
抗原
See all Laminin gamma 1 (LAMC1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- A murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.
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亚型
- IgG2a kappa
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anti-Laminin, gamma 1 (LAMC1) (AA 1300-1609) antibody
LAMC1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) (all Isoforms) antibodyLAMC1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) (AA 1-38) antibodyLAMC1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal M1 unconjugated
anti-Laminin, gamma 1 (LAMC1) (AA 521-772) antibodyLAMC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) (AA 1333-1607) antibodyLAMC1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) (AA 1-38) antibodyLAMC1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E6-B4 unconjugated
anti-Laminin, gamma 1 (LAMC1) (N-Term) antibodyLAMC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) antibodyLAMC1 适用: 小鼠 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) (AA 1566-1579) antibodyLAMC1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Laminin, gamma 1 (LAMC1) (C-Term) antibodyLAMC1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Laminin gamma 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))
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别名
- Laminin gamma 1
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背景
- Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific mAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes.
抗原
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