HSP27 抗体
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北京 101111
Quick Overview for HSP27 抗体 (ABIN3025019)
抗原
See all HSP27 (HSPB1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- HSP27 Antibody (azide and preservative free)
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产品特性
- It recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50 % of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
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亚型
- IgG1, kappa
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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 小鼠, 大鼠, 猴, 绵羊, 小鸡, Chimpanzee WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal G3-1 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, FACS, IP 宿主: 兔 Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Biotin)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 Biotin
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 HRP
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Atto 390)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 Atto 390
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应用备注
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The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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别名
- HSP27
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背景
- It recognizes a 24-27 kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50 % of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
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UniProt
- P04792
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途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
抗原
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