Keratin 5/8 抗体
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北京 101111
Quick Overview for Keratin 5/8 抗体 (ABIN3025013)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 小鸡, 非洲爪蟾
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产品特性
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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纯化方法
- Protein G affinity chromatography
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免疫原
- A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
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亚型
- IgG1 kappa
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anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
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应用备注
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Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.
1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cytokeratin 5/8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Keratin 5/8 (KRT5/8)
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别名
- Cytokeratin 5/8
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背景
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
抗原
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