GRP94 抗体
Quick Overview for GRP94 抗体 (ABIN3024904)
抗原
See all GRP94 (HSP90B1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.
-
亚型
- IgG2a kappa
-
-
-
-
应用备注
-
Titering of the GRP94 antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistology (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
-
别名
- GRP94
-
背景
- Heat shock protein 90 kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]
-
途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
抗原
-