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Nkx2-2 抗体

This 小鼠 单克隆 anti-Nkx2-2 antibody (Clone NX2-294) specifically detects Nkx2-2 in IF, FACS 和 IHC (p). The antibody is reactive with 人, 小鼠, 大鼠 和 小鸡 samples.
产品编号 ABIN3024826
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Nkx2-2 抗体 (ABIN3024826)

抗原

See all Nkx2-2 抗体
Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

适用

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人, 小鼠, 大鼠, 小鸡

宿主

  • 85
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小鼠

克隆类型

  • 92
  • 50
单克隆

标记

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This Nkx2-2 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

NX2-294
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Human NKX2.2 recombinant protein was used as the immunogen for this antibody.

    亚型

    IgG2b kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

    别名

    NKX2.2

    背景

    Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

    基因ID

    4821

    途径

    Dopaminergic Neurogenesis
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