Keratin Acidic (AE1) 抗体
Quick Overview for Keratin Acidic (AE1) 抗体 (ABIN3024694)
抗原
See all Keratin Acidic (AE1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G purified
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免疫原
- Solubilized keratin extract from human stratum corneum
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亚型
- IgG1
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anti-Keratin Acidic (AE1) antibody
AE1 适用: 人 IHC, WB 宿主: 大鼠 Monoclonal OTI1D10 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠, 小鸡, Cow, 小鼠, 兔, 猴, Pig IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal AE1 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人, 大鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IF, IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
anti-Keratin Acidic (AE1) antibodyAE1 适用: 人 IF, IHC (p) 宿主: 兔 Monoclonal RMAK1-1 unconjugated Recombinant Antibody
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody AE1 to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Keratin Acidic (AE1)
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别名
- Acidic Cytokeratin
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背景
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Cytokeratins, also called keratins or simply CKs, are a family of fibrous structural proteins and a major structural component in the outer layer of human skin, as well as hair and nails. There are 20 human epithelial keratins which can be divided into two subfamiles: acidic and basic (more recent human genone sequencing has identified an additional 20 members). The formation of cytokeratin intermediate filaments requires the pairing of at least one acidic and one basic subfamily member. Members of the same keratin subfamily share extended sequence homology while members of different subfamilies show only limited sequence homology.
This antibody is specific for the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), and 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).
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基因ID
- 3858
抗原
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