Melanoma gp100 抗体
Quick Overview for Melanoma gp100 抗体 (ABIN3024239)
抗原
See all Melanoma gp100 (PMEL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 大鼠
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纯化方法
- Protein G affinity chromatography
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免疫原
- Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.
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亚型
- IgG2b kappa
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应用备注
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The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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别名
- Gp100
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背景
- By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
抗原
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