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Melanoma gp100 抗体

This 小鼠 单克隆 antibody specifically detects Melanoma gp100 in IF, FACS 和 IHC (p). It exhibits reactivity toward 人 和 马.
产品编号 ABIN3024239
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Melanoma gp100 抗体 (ABIN3024239)

抗原

See all Melanoma gp100 (PMEL) 抗体
Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

适用

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人, 马

宿主

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小鼠

克隆类型

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单克隆

标记

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This Melanoma gp100 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPM286
  • 无交叉反应

    大鼠

    纯化方法

    Protein G affinity chromatography

    免疫原

    Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

    亚型

    IgG2b kappa
  • 应用备注

    The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the anti-gp100 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

    别名

    Gp100

    背景

    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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