Melanoma gp100 抗体
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- 抗原 See all Melanoma gp100 (PMEL) 抗体
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Melanoma gp100 antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Extract of pigmented melanoma metastases from lymph nodes (HMB45) and recombinant human protein (PMEL/783) were used as the immunogen for the gp100 antibody cocktail.
- 克隆位点
- HMB45-PMEL-783
- 亚型
- IgG
- Top Product
- Discover our top product PMEL Primary Antibody
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anti-Premelanosome Protein (PMEL) antibody
PMEL 适用: 人, 大鼠, 犬, 猴 IF, IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 10E5 unconjugated
anti-Premelanosome Protein (PMEL) (AA 376-502) antibodyPMEL 适用: 人 IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal PMEL-2037 unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IF, IHC, FACS, StM 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyVerified PMEL 适用: 人, 小鼠 IF, WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (N-Term) antibodyPMEL 适用: 人, 马, 大鼠, 小鼠, 犬, Cow, 山羊, 豚鼠, 兔 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (AA 25-467) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, StM 宿主: 兔 Monoclonal PMEL-1825R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) (C-Term) antibodyPMEL 适用: 人, 小鼠 IF, IHC, WB, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-617R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) (AA 1-661) antibodyPMEL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Optimal dilution of the gp100 antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the gp100 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- 别名
- Gp100 (PMEL 产品)
- 别名
- D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody
- 背景
- By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This mAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This mAb also stains Angiomyolipoma (PEComa).
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