beta Actin 抗体 (N-Term)
Quick Overview for beta Actin 抗体 (N-Term) (ABIN3024135)
抗原
See all beta Actin (ACTB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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纯化方法
- Protein A Affinity Chromatography
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免疫原
- A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
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亚型
- IgG
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应用备注
- Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.\. Western blot: 1-3 μg/mL
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- 0.5 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
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- beta Actin (ACTB) (Actin, beta (ACTB))
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别名
- beta Actin
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背景
- Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
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基因ID
- 60
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途径
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
抗原
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