CLIP 抗体 (AA 25-39)
Quick Overview for CLIP 抗体 (AA 25-39) (ABIN3024113)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 25-39
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone were used as the immunogen for this ACTH antibody.
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亚型
- IgG1
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anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 156-176) antibody
CLIP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 156-176) antibodyCLIP 适用: 人 WB 宿主: 小鼠 Monoclonal ABM52H4 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人, 大鼠, 小鼠 IHC (p) 宿主: 兔 Monoclonal CLIP-2040R unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人, 大鼠, 小鼠 IHC (p) 宿主: 兔 Monoclonal CLIP-2040R unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人 FACS, IF, IHC (p), ELISA 宿主: 小鼠 Monoclonal ADCT1-1 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) antibodyCLIP 适用: 人 FACS, IF, WB 宿主: 小鼠 Monoclonal CLIP-1133 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) antibodyCLIP 适用: 人 FACS, IF, WB 宿主: 小鼠 Monoclonal CLIP-1133 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10 mM Citrate Buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: order BSA free format for coating,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE):0.5-1 μg/mL
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CLIP (Corticotropin-Like Intermediate Peptide (CLIP))
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别名
- ACTH / CLIP
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背景
- ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
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基因ID
- 5443
抗原
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