我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

p21 抗体 (AA 43-142)

Name: p21 抗体 (AA 43-142)
Brand: antibodies-online
SKU: ABIN3022006
Availability: OutOfStock

This anti-p21 antibody is a 兔多克隆 antibody detecting p21 in WB 和 IF. Suitable for 人. This Primary Antibody has been cited in 3+ publications.

Western blot analysis of extracts of various cell lines, using CDKN1A antibody. (p21 抗体 (AA 43-142))

Immunofluorescence analysis of HeLa cells using CDKN1A/p21CIP1 antibody (ABIN3022005, ABIN3022006, ABIN3022007 and ABIN6218565) at dilution of 1:100. (p21 抗体 (AA 43-142))

Immunofluorescence analysis of MCF7 cell using CDKN1A antibody. (p21 抗体 (AA 43-142))

Results of p21 gene expression in the glomeruli of type 1 diabetic rats. (p21 抗体 (AA 43-142))

Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (ABIN3022005, ABIN3022006, ABIN3022007 and ABIN6218565) at 1:1000 dilution. (p21 抗体 (AA 43-142))

Immunofluorescence analysis of C6 cells using CDKN1A/p21CIP1 antibody (ABIN3022005, ABIN3022006, ABIN3022007 and ABIN6218565) at dilution of 1:100. (p21 抗体 (AA 43-142))

Immunofluorescence analysis of NIH/3T3 cells using CDKN1A/p21CIP1 antibody (ABIN3022005, ABIN3022006, ABIN3022007 and ABIN6218565) at dilution of 1:100. (p21 抗体 (AA 43-142))

7 images

(3 references)

产品编号 ABIN3022006

发货至: 中国

Datasheet as PDF

Quick Overview for p21 抗体 (AA 43-142) (ABIN3022006)

抗原

See all p21 (CDKN1A) 抗体

p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

适用

人

宿主

兔

克隆类型

多克隆

标记

This p21 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

抗原表位
36

30

16

15

15

10

9

8

8

8

8

7

7

7

6

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 43-142

序列

QEARERWNFD FVTETPLEGD FAWERVRGLG LPKLYLPTGP RRGRDELGGG RRPGTSPALL QGTAEEDHVD LSLSCTLVPR SGEQAEGSPG GPGDSQGRKR

交叉反应

人, 小鼠, 大鼠

产品特性

Polyclonal Antibodies

纯化方法

Affinity purification

免疫原

Recombinant fusion protein containing a sequence corresponding to amino acids 43-142 of human CDKN1A/p21CIP1 (NP_000380.1).

亚型

IgG
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 65-164) antibody
CDKN1A 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2F1 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody
KO Validated CDKN1A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-164) antibody
CDKN1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-100) antibody
CDKN1A 适用: Synthetic WB, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody
CDKN1A 适用: 人, Non-Human Primate ELISA, IHC (p), ICC 宿主: 小鼠 Monoclonal WA-1 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 21-100) antibody
CDKN1A 适用: 人, 小鼠, 大鼠, 绵羊 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibody
CDKN1A 适用: 人 WB, IHC, IF, IHC (f) 宿主: 小鼠 Monoclonal CIP1-823 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody
CDKN1A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibody
CDKN1A 适用: 人 WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) antibody
CDKN1A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB,1:500 - 1:2000,IF,1:50 - 1:200

限制

仅限研究用
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Guo, Zhao, Fei, Zhao, Zheng, Su, Wu, Li, Chang: "Dicer1 downregulation by multiple myeloma cells promotes the senescence and tumor-supporting capacity and decreases the differentiation potential of mesenchymal stem cells." in: Cell death & disease, Vol. 9, Issue 5, pp. 512, (2018) (PubMed).

Wu, Wang, Luo, Zhang, Wang, Zhu, Gao, Du, Yang: "Metabolic perturbation of epigenome by inhibiting S-adenosylhomocysteine hydrolase elicits senescence through DNA damage response in hepatoma cells." in: Tumour biology, Vol. 39, Issue 5, pp. 1010428317699117, (2017) (PubMed).

Li, Li, Li, Cui, Li, Guo, Han, Liu, Sun: "Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions." in: Journal of diabetes research, Vol. 2016, pp. 3853242, (2017) (PubMed).
抗原

p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

别名

CDKN1A

背景

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.,CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1,CDKN1A,Epigenetics & Nuclear Signaling,DNA Damage & Repair,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Cell cycle inhibitors,G1/S Checkpoint,G2/M DNA Damage Checkpoint,Endocrine & Metabolism,AMPK Signaling Pathway,Immunology & Inflammation,Stem Cells,CDKN1A

分子量

21 kDa

基因ID

1026

UniProt

P38936

途径

p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy