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GFP 抗体 (AA 1-100)

This anti-GFP antibody is a 小鼠 单克隆 antibody detecting GFP in WB 和 IF. Suitable for Aequorea victoria. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN3020573
发货至: 中国

Quick Overview for GFP 抗体 (AA 1-100) (ABIN3020573)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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小鼠

克隆类型

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单克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    AA 1-100

    序列

    MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF

    产品特性

    Tag Antibodies

    纯化方法

    Affinity purification

    免疫原

    A synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein.

    亚型

    IgG
  • 应用备注

    WB,1:2000 - 1:5000,IF,1:50 - 1:100

    限制

    仅限研究用
  • 缓冲液

    PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid freeze / thaw cycles

    储存条件

    -20 °C

    储存方法

    Store at -20°C. Avoid freeze / thaw cycles.
  • Xu, Liu, Zha, Fan, Zhang, Li, Xiao: "A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein." in: PLoS pathogens, Vol. 14, Issue 8, pp. e1007259, (2019) (PubMed).

    Yang, Yang, Sun, Chen, Xu, Lai, Li, Wang, Bhattarai, Xiao, Sun, Zhu, Ma, Adhikari, Sun, Hao, Zhang, Huang, Huang, Jiang, Zhao, Wang, Sun, Yang: "5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m5C reader." in: Cell research, Vol. 27, Issue 5, pp. 606-625, (2018) (PubMed).

    Zhang, Lu, Liu, Cui, Wang: "Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease." in: Aging, Vol. 8, Issue 2, pp. 272-90, (2016) (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. It has been used in modified forms to make biosensors, and many animals have been created that express GFP, which demonstrates a proof of concept that a gene can be expressed throughout a given organism, in selected organs, or in cells of interest. GFP can be introduced into animals or other species through transgenic techniques, and maintained in their genome and that of their offspring. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.,GFP,GFP tag,GFP-tag,GFP

    分子量

    27 kDa
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