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Fibronectin 1 抗体 (Biotin)

This anti-Fibronectin 1 antibody is a 兔 多克隆 antibody detecting Fibronectin 1 in WB, ELISA, IHC, IP 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, Cow 和 猴.
产品编号 ABIN298210
发货至: 中国

Quick Overview for Fibronectin 1 抗体 (Biotin) (ABIN298210)

抗原

See all Fibronectin 1 (FN1) 抗体
Fibronectin 1 (FN1)

适用

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人, 小鼠, 大鼠, Cow, 猴

宿主

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克隆类型

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多克隆

标记

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This Fibronectin 1 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross- reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible

    纯化方法

    Immunoaffinity purified

    免疫原

    Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single- band purity was assessed by SDS-PAGE.
  • 应用备注

    Approved: ELISA (1:4000 - 1:8000), IHC, IHC-Fr (1:50 - 1:200), IP, WB (1:10000)

    Usage: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    deionized water

    浓度

    Lot specific

    缓冲液

    Lyophilized, 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • 抗原

    Fibronectin 1 (FN1)

    别名

    FN1 / Fibronectin

    背景

    Name/Gene ID: FN1

    Synonyms: FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF

    基因ID

    2335

    UniProt

    P02751

    途径

    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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