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GFP 抗体 (HRP)

This anti-GFP antibody is a 山羊 多克隆 antibody detecting GFP in WB, ELISA, IHC 和 IF. Suitable for Aequorea victoria.
产品编号 ABIN297131
发货至: 中国

Quick Overview for GFP 抗体 (HRP) (ABIN297131)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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山羊

克隆类型

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多克隆

标记

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This GFP antibody is conjugated to HRP

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 特异性

    Recognizes wild type, recombinant and enhanced GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins.

    纯化方法

    Immunoaffinity purified

    免疫原

    GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

    Type of Immunogen: Fusion protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA (1:10000 - 1:50000), IF, IHC (1:500 - 1:3000), WB (1:1000 - 1:5000)

    Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000-1:50000. Sandwich or capture. Recognizes wild type, recombinant and enhanced forms of GFP. Immunohistochemistry: 1:500-1:3000. Immunofluorescence: Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic. (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Western Blot: 1:1000-1:5000. Detects a 42kD band. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Aequorea victoria

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

    储存液

    Gentamicin sulfate

    注意事项

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    Name/Gene ID: GFP

    Synonyms: GFP

    UniProt

    P42212
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