MAPK7 抗体 (pThr218, Tyr220)
Quick Overview for MAPK7 抗体 (pThr218, Tyr220) (ABIN296169)
抗原
See all MAPK7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 213-224, pThr218, Tyr220
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特异性
- Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (AA 1-310) antibody
MAPK7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (AA 185-419) antibodyMAPK7 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (AA 776-805) antibodyMAPK7 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (pSer496) antibodyMAPK7 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (pSer731), (pThr733) antibodyMAPK7 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (AA 401-500) antibodyMAPK7 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) antibodyMAPK7 适用: 人, 小鼠, 大鼠 WB, IF (cc), FACS, IHC (p) 宿主: 兔 Monoclonal 8D8 unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (AA 1-310) antibodyMAPK7 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 7 (MAPK7) (AA 560-793) antibodyMAPK7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: WB
Usage: Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).
Not recommended for: IHC-P -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))
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别名
- MAPK7 / ERK5
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背景
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Name/Gene ID: MAPK7
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK7, Big MAP kinase 1, BMK-1, Erk4, ERK5, MAPK 7, PRKM7, BMK1, BMK1 kinase, ERK-5, MAP kinase 7 -
基因ID
- 5598
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UniProt
- Q13164
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling
抗原
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