Histone H2B 抗体 (AA 111-125)
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北京 101111
Quick Overview for Histone H2B 抗体 (AA 111-125) (ABIN291581)
抗原
See all Histone H2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 111-125
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特异性
- A 15 kD band is observed. This is routinely quality controlled in human lysates.
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预测反应
- Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).
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纯化方法
- Protein G purified
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免疫原
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This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B, GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Histone H2B (acLys12) antibody
适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK20ac) antibody适用: 人 WB, ELISA, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (acLys5) antibody适用: 人 WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (AA 1-126) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Histone H2B (acLys120) antibody适用: 人 WB, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B antibody适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A6 unconjugated
anti-Histone H2B (H2BK15ac) antibody适用: 人 WB, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK116ac) antibody适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone H2B (H2BK11ac) antibody适用: 人 WB, ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Histone H2B
抗原
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