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Histone H2B 抗体 (H2BK120ac)

Name: Histone H2B 抗体 (H2BK120ac)
SKU: ABIN2668336
Availability: OutOfStock

There are 2+ publications for this product available. The 兔多克隆 anti-Histone H2B antibody is suitable to detect Histone H2B in samples from 人. It has been validated for WB, IF, ChIP, DB 和 ChIP-seq.

Histone H2B acetyl Lys120 pAb tested by Western blot. (Histone H2B 抗体 (H2BK120ac))

ChIP-seq

Histone H2B acetyl Lys120 pAb tested by dot blot analysis. (Histone H2B 抗体 (H2BK120ac))

ChIP

4 images

(2 references)

产品编号 ABIN2668336

发货至: 中国

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Quick Overview for Histone H2B 抗体 (H2BK120ac) (ABIN2668336)

抗原

See all Histone H2B 抗体

Histone H2B

适用

人

宿主

兔

克隆类型

多克隆

标记

This Histone H2B antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Discover our top product Histone H2B Primary Antibody

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H2BK120ac

原理

Histone H2BK120ac antibody (pAb)

纯化方法

Unpurified

免疫原

This Histone H2B acetyl Lys120 antibody was raised against a peptide including acetyl-lysine 120 of human histone H2B.
anti-Histone H2B (acLys12) antibody
适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B (H2BK20ac) antibody
适用: 人 WB, ELISA, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B (H2BK5ac) antibody
适用: 人 WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B (AA 1-126) antibody
适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B antibody
适用: 人, 小鼠, 大鼠 WB, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Histone H2B antibody
适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 3A6 unconjugated

anti-Histone H2B (H2BK15ac) antibody
适用: 人 WB, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B (H2BK116ac) antibody
适用: 人 WB, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B (H2BK11ac) antibody
适用: 人 WB, ELISA, IHC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Histone H2B (H2BK120ac) antibody
适用: 人 ELISA, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 10 µL per ChIP ChIP-Seq: 5 µL each WB*: 1:1,000 - 1:2,000 dilution IF: 1:500 dilution The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H2B acetyl Lys120 antibody was validated for ChIP-Seq in the study (see reference). *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Alvarez-Saavedra, De Repentigny, Lagali, Raghu Ram, Yan, Hashem, Ivanochko, Huh, Yang, Mears, Todd, Corcoran, Bassett, Tokarew, Kokavec, Majumder, Ioshikhes, Wallace, Kothary, Meshorer, Stopka et al.: "Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. ..." in: Nature communications, Vol. 5, pp. 4181, (2014) (PubMed).

Gatta, Mantovani: "Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 11, pp. 2149-59, (2011) (PubMed).
抗原

Histone H2B

背景

Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.

分子量

15 kDa

基因ID

8348

NCBI登录号

NP_778225