Affinity purified Goat polyclonal IFN beta antibody Bioactivity: The exact concentration of antibody required to neutralize human interferon beta activity is dependent on the cytokine concentration, cell type, growth conditions and type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this antibody on human (HeLa/EMCV) cells is approx. 0.05- 0.2 µg/mL in the presence of 10ng/mL of human interferon beta, based on the anti-viral assay. The specific conditions are described in the figure legends.
纯化方法
Purified
免疫原
IFN beta antibody was raised in goat using human interferon beta as the immunogen.
Optimal conditions should be determined byt he investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in sterile PBS.
浓度
Lot specific
缓冲液
Lyophilized from PBS with 5 % trehalose.
注意事项
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
IFNB1
(Interferon, beta 1, Fibroblast (IFNB1))
别名
IFN beta
背景
Interferons (IFNs) are proteins made and released by lymphocytes in response to the presence of pathogens-such as viruses, bacteria, or parasites-or tumor cells. They allow communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors.