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GFP 抗体

This anti-GFP antibody is a 山羊 多克隆 antibody detecting GFP in WB, ELISA 和 IHC. Suitable for Aequorea victoria. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN289429
发货至: 中国

Quick Overview for GFP 抗体 (ABIN289429)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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山羊

克隆类型

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多克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 交叉反应 (详细)

    Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml

    纯化方法

    Affinity chromatography purified

    过滤

    Filtered

    免疫原

    GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.
  • 应用备注

    ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
    Optimal conditions should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze/thaw cycles.
    Dilute only prior to immediate use.

    储存条件

    4 °C/-20 °C

    储存方法

    Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
  • Buser, Drubin: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).

    Buser, McDonald: "Correlative GFP-immunoelectron microscopy in yeast." in: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
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