GFP 抗体
Quick Overview for GFP 抗体 (ABIN289429)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
- 
    
- 
                                            
交叉反应 (详细)
 - Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml
 - 
                                            
纯化方法
 - Affinity chromatography purified
 - 
                                            
过滤
 - Filtered
 - 
                                            
免疫原
 - GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.
 
 - 
                                            
 - 
    
 - 
    
- 
                                            
应用备注
 - 
                        ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator. - 
                                            
限制
 - 仅限研究用
 
 - 
                                            
 - 
    
- 
                                            
状态
 - Liquid
 - 
                                            
浓度
 - Lot specific
 - 
                                            
缓冲液
 - Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.
 - 
                                            
储存液
 - Sodium azide
 - 
                                            
注意事项
 - This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
 - 
                                            
注意事项
 - 
                        Avoid repeated freeze/thaw cycles. 
Dilute only prior to immediate use. - 
                                            
储存条件
 - 4 °C/-20 °C
 - 
                                            
储存方法
 - Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.
 
 - 
                                            
 - 
    
- 
                                            : "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling."    in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013)         (PubMed).        
: "Correlative GFP-immunoelectron microscopy in yeast." in: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).
 
 - 
                                            : "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling."    in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013)         (PubMed).        
 - 
    
- GFP (Green Fluorescent Protein (GFP))
 - 
                                            
别名
 - GFP
 - 
                                            
背景
 - Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
 
抗原
 -