Minimum cross reactivity to Human, Mouse and Rat serum proteins1 mg/ml
纯化方法
Affinity chromatography purified
免疫原
GFP antibody (HRP) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.
ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000 Optimal conditions should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
浓度
Lot specific
缓冲液
Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.
储存液
Without preservative
注意事项
Avoid repeated freeze/thaw cycles. Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Dilute only prior to immediate use.
储存条件
4 °C/-20 °C
储存方法
Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
抗原
GFP
(Green Fluorescent Protein (GFP))
别名
GFP
背景
Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.