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Cyclin A 抗体

Name: Cyclin A 抗体
Brand: antibodies-online
SKU: ABIN2855543
Availability: OutOfStock

This anti-Cyclin A antibody (ABIN2855543) is a Rabbit Polyclonal antibody detecting Cyclin A in WB, IF, IP, IHC (p), ICC. Suitable for Human.

WB Image Cyclin A2 antibody detects CCNA2 protein by Western blot analysis. (Cyclin A 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using cyclin A , antibody at 1:500 dilution. (Cyclin A 抗体)

IHC

WB Image Cyclin A2 antibody detects Cyclin A2 protein by western blot analysis. (Cyclin A 抗体)

IP Image Cyclin A2 antibody immunoprecipitates cyclin A2 protein in IP experiments. (Cyclin A 抗体)

ICC/IF Image Cyclin A2 antibody detects Cyclin A2 protein at cytoplasm and nucleus by immunofluorescent analysis. (Cyclin A 抗体)

p-Akt and p-NFκB levels of C2-ceramide-treated H1299 lung cancer cells. (Cyclin A 抗体)

8 images

(5 references)

产品编号 ABIN2855543

发货至: 中国

Datasheet as PDF

Quick Overview for Cyclin A 抗体 (ABIN2855543)

抗原

See all Cyclin A (CCNA2) 抗体

Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

适用

人

宿主

兔

克隆类型

多克隆

标记

This Cyclin A antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

质量等级

KO Validated

特异性

IP/MS validation was supported by references (PMID:30377384)

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to Cyclin A2 (cyclin A2)
Cyclin A2 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human Cyclin A2. The exact sequence is proprietary.

亚型

IgG
anti-Cyclin A2 (CCNA2) (AA 105-233) antibody
CCNA2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6B4D11 unconjugated

anti-Cyclin A2 (CCNA2) (AA 1-200) antibody
CCNA2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Cyclin A2 (CCNA2) (AA 51-84) antibody
CCNA2 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Cyclin A2 (CCNA2) (AA 375-431) antibody
CCNA2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin A2 (CCNA2) antibody
CCNA2 适用: 人, 小鼠, Cow, Green Monkey, Mink IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal E67 unconjugated

anti-Cyclin A2 (CCNA2) antibody
CCNA2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin A2 (CCNA2) (C-Term) antibody
CCNA2 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin A2 (CCNA2) (AA 51-84), (N-Term) antibody
CCNA2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14617 unconjugated

anti-Cyclin A2 (CCNA2) (C-Term) antibody
CCNA2 适用: 人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 犬, 马, 豚鼠, Saccharomyces cerevisiae, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin A2 (CCNA2) antibody
CCNA2 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Neuro2A , PC-12 , IMR32 , SK-N-AS , HeLa(100 μg/ml Bleomycin treatment for 24 hr)
Validation: IP/MS, KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.73 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Honda, Inui: "PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression." in: Scientific reports, Vol. 10, Issue 1, pp. 1140, (2020) (PubMed).

Ogawa, Yaginuma, Nakatomi, Nakajima, Tada-Shigeyama, Addison, Urata, Matsubara, Watanabe, Matsuo, Sato, Honda, Hikiji, Watanabe, Kokabu: "Transducin-like enhancer of split 3 regulates proliferation of melanoma cells via histone deacetylase activity." in: Oncotarget, Vol. 10, Issue 3, pp. 404-414, (2019) (PubMed).

Fu, Xu, Chen, Li, Zhao, Chen: "Microarray analysis reveals Tmub1 as a cell cycle-associated protein in rat hepatocytes." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 4337-4344, (2018) (PubMed).

Li, Wu, Lee, Li, Hsieh, Ho, Ho, Chen, Wang, Yen, Yang, Chen: "Hinokitiol induces DNA damage and autophagy followed by cell cycle arrest and senescence in gefitinib-resistant lung adenocarcinoma cells." in: PLoS ONE, Vol. 9, Issue 8, pp. e104203, (2015) (PubMed).

Lin, Chou, Lee, Chen, Fong, Chang, Huang, Wu, Chang, Wang, Chiu: "The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB." in: Cancer cell international, Vol. 14, Issue 1, pp. 1, (2014) (PubMed).
抗原

Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

别名

cyclin A2

背景

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Cellular Localization: Nucleus , Cytoplasm

分子量

49 kDa

基因ID

890

UniProt

P20248

途径

PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA