我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

BTRC 抗体

Name: BTRC 抗体
Brand: antibodies-online
SKU: ABIN2855453
Availability: OutOfStock

This anti-BTRC antibody is a 兔多克隆 antibody detecting BTRC in WB 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 1 publication.

WB Image Sample (30 ug of whole cell lysate) A: 293T 7. (BTRC 抗体)

IHC-P Image beta-TrCP antibody [N1C1] detects beta-TrCP protein at nucleus on rat fore brain by immunohistochemical analysis. (BTRC 抗体)

IHC

Hypoxia induces NED of LNCaP cells concomitant with down-regulation REST protein levels but not REST mRNA(A) LNCaP cells were treated with hypoxia (2 % O2) for 3 days. (BTRC 抗体)

3 images

(1 reference)

产品编号 ABIN2855453

发货至: 中国

Quick Overview for BTRC 抗体 (ABIN2855453)

抗原

See all BTRC 抗体

BTRC (beta-Transducin Repeat Containing (BTRC))

适用

人

宿主

兔

克隆类型

多克隆

标记

This BTRC antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product BTRC Primary Antibody

交叉反应

人, 大鼠

产品特性

Rabbit polyclonal antibody to beta-TrCP (beta-transducin repeat containing)
beta-TrCP antibody [N1C1]

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human beta-TrCP. The exact sequence is proprietary.

亚型

IgG
anti-beta-Transducin Repeat Containing (BTRC) (AA 24-151) antibody
BTRC 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4C5D8 unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 50-340) antibody
BTRC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 397-448) antibody
BTRC 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 1-605) antibody
BTRC 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (N-Term) antibody
BTRC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 52-354) antibody
BTRC 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 2H2 unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 17-52), (N-Term) antibody
BTRC 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16275 unconjugated

anti-beta-Transducin Repeat Containing (BTRC) antibody
BTRC 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 701-1000) antibody
BTRC 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-beta-Transducin Repeat Containing (BTRC) (AA 52-354) antibody
BTRC 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1F10 unconjugated

应用备注

WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Lin, Chang, Lee, Campbell, Wang, Shen, Chung, Chang, Chiu, Pan, Lin, Chu, Kung, Cheng, Chang: "REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling." in: Oncotarget, Vol. 7, Issue 18, pp. 26137-51, (2018) (PubMed).
抗原

BTRC (beta-Transducin Repeat Containing (BTRC))

别名

beta-transducin repeat containing E3 ubiquitin protein ligase

背景

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class, in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

Cellular Localization: Cytoplasm

分子量

69 kDa

基因ID

8945

UniProt

Q9Y297

途径

Cell Division Cycle, Hedgehog Signaling