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Calnexin 抗体 (Internal Region)

Cited in 3+ publications. The 兔 多克隆 anti-Calnexin antibody (ABIN2855124) specifically detects Calnexin in WB, IF, ICC 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN2855124
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Calnexin 抗体 (Internal Region) (ABIN2855124)

抗原

See all Calnexin (CANX) 抗体
Calnexin (CANX)

适用

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人, 小鼠, 大鼠

宿主

  • 135
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克隆类型

  • 122
  • 61
多克隆

标记

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This Calnexin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 43
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    Internal Region

    交叉反应

    小鸡, 犬, 非洲爪蟾, 斑马鱼, 牛

    交叉反应 (详细)

    Chicken (91 %), Dog (94 %), Xenopus laevis (88 %), Zebrafish (82 %), Bovine (94 %)

    产品特性

    Rabbit Polyclonal antibody to Calnexin (calnexin)
    Calnexin antibody [N3C2], Internal

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

    说明

    Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , PC-12 , Rat2

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.87 mg/mL

    缓冲液

    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • de Jong, Murphy, Mäger, Willms, Garcia-Guerra, Gitz-Francois, Lefferts, Gupta, Steenbeek, van Rheenen, El Andaloussi, Schiffelers, Wood, Vader: "A CRISPR-Cas9-based reporter system for single-cell detection of extracellular vesicle-mediated functional transfer of RNA." in: Nature communications, Vol. 11, Issue 1, pp. 1113, (2020) (PubMed).

    Ilahibaks, Lei, Mol, Deshantri, Jiang, Schiffelers, Vader, Sluijter: "Biofabrication of Cell-Derived Nanovesicles: A Potential Alternative to Extracellular Vesicles for Regenerative Medicine." in: Cells, Vol. 8, Issue 12, (2020) (PubMed).

    Sever, Vo, Bols, Dixon: "Rainbow trout (Oncorhynchus mykiss) contain two calnexin genes which encode distinct proteins." in: Developmental and comparative immunology, Vol. 42, Issue 2, pp. 211-9, (2014) (PubMed).

  • 抗原

    Calnexin (CANX)

    别名

    Calnexin

    背景

    This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.

    Cellular Localization: Endoplasmic reticulum membrane , Melanosome

    分子量

    68 kDa

    基因ID

    821

    途径

    MAPK Pathway, Thyroid Hormone Synthesis
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