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MTOR 抗体 (C-Term)

Name: MTOR 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2855088
Availability: OutOfStock

This anti-MTOR antibody is a 兔多克隆 antibody detecting MTOR in WB, IF, IP, IHC (p), ICC 和 ChIP. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 5+ publications.

WB Image mTOR antibody detects mTOR protein by western blot analysis. (MTOR 抗体 (C-Term))

ChIP

IHC-P Image mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis. (MTOR 抗体 (C-Term))

IHC

IP Image Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term, Western blot analysis was performed using mTOR antibody [C3], C-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (MTOR 抗体 (C-Term))

IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. (MTOR 抗体 (C-Term))

IHC

ICC/IF Image mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunofluorescent analysis. (MTOR 抗体 (C-Term))

IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. (MTOR 抗体 (C-Term))

IHC

IP Image mTOR antibody immunoprecipitates mTOR protein in IP experiments. (MTOR 抗体 (C-Term))

MIF facilitates autophagy induction in endothelial cells. (MTOR 抗体 (C-Term))

BMP4 inhibits triglyceride accumulation through regulating the genes involved in lipid metabolism and members of mTORC1 signaling pathway in hepatocytes. (MTOR 抗体 (C-Term))

BMP4 down-regulates the mTORC1 signaling pathway in mouse liver. (MTOR 抗体 (C-Term))

IHC

Exogenous BMP4 attenuates the development and progression of NAFLD by suppressing the mTORC1 signaling pathway. (MTOR 抗体 (C-Term))

IHC

15 images

(5 references)

产品编号 ABIN2855088

发货至: 中国

Datasheet as PDF

Quick Overview for MTOR 抗体 (C-Term) (ABIN2855088)

抗原

See all MTOR (mTOR) 抗体

MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This MTOR antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Discover our top product mTOR Primary Antibody

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C-Term

产品特性

Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

亚型

IgG
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody
mTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibody
mTOR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2400-2500) antibody
mTOR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) antibody
mTOR 适用: 人, 小鼠, 大鼠, 鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2311-2529) antibody
mTOR 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H5A5 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibody
mTOR 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB13347 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibody
mTOR 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2481) antibody
mTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2448) antibody
mTOR 适用: 人, 小鼠, 大鼠, Cow, 鱼 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibody
mTOR 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal D7 unconjugated

应用备注

Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

说明

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Wang, Cao, Shu, Zhu, Peng, Ran, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes." in: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1399-1412, (2020) (PubMed).

Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

Peng, Chen, Wang, Zhu, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes." in: Aging, Vol. 11, Issue 23, pp. 11520-11540, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).
抗原

MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

别名

mTOR

背景

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side

分子量

289 kDa

基因ID

2475

途径

PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect