HIF1A 抗体 (AA 432-528)

HIF1A抗体详情 (hide)

抗原 See all HIF1A 抗体

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

抗原表位 All epitopes for HIF1A 抗体

• C-Term 22
• AA 329-530 20
• AA 341-450 16
• AA 661-760 16
• AA 735-762 14
• N-Term 8
• AA 547-577 7
• AA 530-825 6
• AA 579-826 6
• AA 218-506 5
• AA 432-528 5
• AA 238-389 4
• AA 35-155 4
• AA 575-826 4
• AA 216-337 3
• AA 45-180 3
• AA 588-836 3
• AA 641-785 3
• AA 641-810 3
• AA 703-732 3
• AA 717-826 3
• Isoform 1 3
• AA 1-30 2
• AA 1-735 2
• AA 220-349 2
• AA 400-550 2
• AA 433-826 2
• AA 470-590 2
• AA 519-547 2
• AA 728-757 2
• AA 776-826 2
• AA 790-830 2
• Center 2
• Internal Region 2
• AA 1-31 1
• AA 216-336 1
• AA 239-530 1
• AA 328-377 1
• AA 350-400 1
• AA 400-450 1
• AA 520-550 1
• AA 530-826 1
• AA 550-600 1
• AA 585-824 1
• AA 670-826 1
• AA 755-804 1
• AA 817-821 1
• AA 833-850 1
• Lys377 1
• Middle Region 1

AA 432-528

适用 All reactivities for HIF1A 抗体

• Human 178
• Mouse 86
• Rat 82
• Cow 24
• Rabbit 22
• Chicken 20
• Dog 14
• Monkey 12
• Goat 9
• Horse 8
• Pig 7
• Guinea Pig 6
• Hamster 6
• Zebrafish (Danio rerio) 5
• Sheep 3
• Avian 2
• Bat 2
• Cat 2
• Donkey 2
• Primate 2
• Chimpanzee 1
• Ferret 1
• Gibbon 1
• Orang-Utan 1
• Protozoa 1
• Saccharomyces cerevisiae 1
• Xenopus laevis 1

人, 小鼠, 大鼠, 兔, 猴, 绵羊

宿主 All hosts for HIF1A 抗体

• Rabbit 167
• Mouse 55
• Goat 6

小鼠

Compatible Secondaries

克隆类型 All clonalities for HIF1A 抗体

• Polyclonal 171
• Monoclonal 57

单克隆

标记 All conjugates for HIF1A 抗体

• 非结合性 120
• Biotin 29
• FITC 18
• HRP 8
• PE 6
• APC 4
• Alkaline Phosphatase (AP) 4
• Alexa Fluor 350 2
• Alexa Fluor 488 2
• Alexa Fluor 555 2
• Alexa Fluor 594 2
• Alexa Fluor 647 2
• Alexa Fluor 680 2
• Alexa Fluor 750 2
• Cy3 2
• Cy5 2
• Cy5.5 2
• Cy7 2
• Magnetic Particles 2
• Sepharose 2
• Atto 390 1
• Atto 488 1
• Atto 594 1
• CF®405S 1
• CF®488A 1
• CF®568 1
• CF®594 1
• CF®640R 1
• CF®647 1
• CF®680 1
• CF®680R 1
• CF®770 1
• PerCP 1

This HIF1A antibody is un-conjugated

应用范围 All applications for HIF1A 抗体

• Western Blotting (WB) 170
• Immunohistochemistry (IHC) 89
• ELISA 76
• Immunofluorescence (IF) 60
• Flow Cytometry (FACS) 53
• Immunocytochemistry (ICC) 39
• Immunoprecipitation (IP) 39
• Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29
• Immunofluorescence (Cultured Cells) (IF (cc)) 26
• Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26
• Immunofluorescence (fixed cells) (IF/ICC) 13
• Immunohistochemistry (Frozen Sections) (IHC (fro)) 8
• Chromatin Immunoprecipitation (ChIP) 7
• Immunochromatography (IC) 7
• ChIP DNA-Sequencing (ChIP-seq) 4
• Proximity Ligation Assay (PLA) 3
• Coating (Coat) 3
• Enzyme Immunoassay (EIA) 2
• Gel Shift (GS) 2
• Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2
• Fluorescence Microscopy (FM) 1
• Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

特异性

Reacts with human HIF-1 alpha

交叉反应

小鼠, 牛, 兔, 绵羊

交叉反应 （详细）

Cross reacts with proteins from monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret.

纯化方法

Purified (protein A)

免疫原

Fusion protein containing amino acids 432-528 of human HIF-1alpha

克隆位点

H1alpha67

亚型

IgG2b

Alternatives (show)

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 341-450) antibody

HIF1A 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732), (C-Term) antibody

HIF1A 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 218-506) antibody

HIF1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 575-826) antibody

HIF1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody

HIF1A 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB14548 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 519-547) antibody

HIF1A 适用: 人, 小鼠 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 728-757) antibody

HIF1A 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 432-528) antibody

HIF1A 适用: 人, 小鼠, 大鼠, Cow, 猴, Pig, 绵羊, 雪貂 WB, IHC, ELISA, IF, IP, ICC, FACS, ChIP, GS 宿主: 小鼠 Monoclonal H1alpha67 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 329-530) antibody

HIF1A 适用: 人, 小鼠, 大鼠, Cow, 犬 WB, IHC, IF, IP, ICC 宿主: 小鼠 Monoclonal ESEE122 unconjugated

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 703-732) antibody

HIF1A 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

使用细节 (hide)

应用备注

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ChIP(1:10 - 1:500) GS(1:1 - 1:100) ICC/IF(1:50) IHC(1:20 - 1:50) IP(1:10 - 1:500) WB: (1:500)

限制

仅限研究用

贮存及处理 (hide)

状态

Liquid

浓度

1.5 mg/mL

缓冲液

Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 4°C. Do not freeze.

HIF1A目标详情 (hide)

抗原

HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

别名

Hypoxia-inducible factor 1-alpha (HIF1A 产品)

别名

hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody

背景

HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis.This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 alpha is involved in B-1 B cell homeostasis, Notch signaling pathway and many other biological processes.

基因ID

3091

UniProt

Q16665

途径

Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect