Sonic Hedgehog 抗体 (N-Term)
Quick Overview for Sonic Hedgehog 抗体 (N-Term) (ABIN2781775)
抗原
See all Sonic Hedgehog (SHH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- RCLLLVLVSS LLVCSGLACG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT
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预测反应
- Cow: 93%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
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产品特性
- This is a rabbit polyclonal antibody against SHH. It was validated on Western Blot and immunohistochemistry.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human SHH
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anti-Sonic Hedgehog (SHH) (AA 21-120) antibody
SHH 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 26-161) antibodySHH 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H4 unconjugated
anti-Sonic Hedgehog (SHH) (C-Term) antibodyVerified SHH 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 26-161) antibodySHH 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 36-193) antibodySHH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 36-193) antibodySHH 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Sonic Hedgehog (SHH) (AA 58-91), (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB52387 unconjugated
anti-Sonic Hedgehog (SHH) (AA 24-197) antibodySHH 适用: 人, 大鼠, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 10H6 unconjugated
anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 237-399) antibodySHH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 462 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Sonic Hedgehog (SHH)
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别名
- SHH
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背景
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SHH is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE). It is also thought that mutations in its gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.This gene, which is expressed only during embryogenesis, encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.
Alias Symbols: HHG1, HLP3, HPE3, SMMCI, TPT, TPTPS, MCOPCB5
Protein Interaction Partner: UBC, SEL1L, DERL2, DERL1, SYVN1, HHIP, PTCH2, PTCH1, SHH, ADCYAP1, GAS1,
Protein Size: 462 -
分子量
- 28 kDa
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基因ID
- 6469
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NCBI登录号
- NM_000193, NP_000184
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UniProt
- Q15465
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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